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转录因子HNF1A、HNF4A和FOXA2调节肝细胞蛋白质N-糖基化 Article
Vedrana Vičić Bočkor,Nika Foglar,Goran Josipović,Marija Klasić,Ana Vujić,Branimir Plavša,Toma Keser,Samira Smajlović,Aleksandar Vojta,Vlatka Zoldoš
《工程(英文)》 2024年 第32卷 第1期 页码 58-69 doi: 10.1016/j.eng.2023.09.019
Hepatocyte nuclear factor 1 alpha (HNF1A), hepatocyte nuclear factor 4 alpha (HNF4A), and forkhead box protein A2 (FOXA2) are key transcription factors that regulate a complex gene network in the liver, creating a regulatory transcriptional loop. The Encode and ChIP-Atlas databases identify the recognition sites of these transcription factors in many glycosyltransferase genes. Our in silico analysis of HNF1A, HNF4A, and FOXA2 binding to the 10 candidate glyco-genes studied in this work confirms a significant enrichment of these transcription factors specifically in the liver. Our previous studies identified HNF1A as a master regulator of fucosylation, glycan branching, and galactosylation of plasma glycoproteins. Here, we aimed to functionally validate the role of the three transcription factors on downstream glyco-gene transcriptional expression and the possible effect on glycan phenotype. We used the state-of-the-art clustered regularly interspaced short palindromic repeats/dead Cas9 (CRISPR/dCas9) molecular tool for the downregulation of the HNF1A, HNF4A, and FOXA2 genes in HepG2 cells—a human liver cancer cell line. The results show that the downregulation of all three genes individually and in pairs affects the transcriptional activity of many glyco-genes, although downregulation of glyco-genes was not always followed by an unambiguous change in the corresponding glycan structures. The effect is better seen as an overall change in the total HepG2 N-glycome, primarily due to the extension of biantennary glycans. We propose an alternative way to evaluate the N-glycome composition via estimating the overall complexity of the glycome by quantifying the number of monomers in each glycan structure. We also propose a model showing feedback loops with the mutual activation of HNF1A–FOXA2 and HNF4A–FOXA2 affecting glyco-genes and protein glycosylation in HepG2 cells.
关键词: Clustered regularly interspaced short palindromic repeats/dead Cas9 (CRISPR/dCas9) Epigenetics Hepatocyte nuclear factor 1 alpha (HNF1A) Hepatocyte nuclear factor 4 alpha (HNF4A) Forkhead box protein A2 (FOXA2) N-glycosylation HepG2 cells
OUYANG Shan, ZHANG Qinghua, QIAO Fuyuan
《医学前沿(英文)》 2007年 第1卷 第4期 页码 386-389 doi: 10.1007/s11684-007-0075-6
关键词: pregnancy concentration possible relationship responsible hepatocyte
《医学前沿(英文)》 2023年 第17卷 第3期 页码 476-492 doi: 10.1007/s11684-022-0966-6
关键词: EIF4A1 G-quadruplex hepatocellular carcinoma tRNA-derived small RNA translation initiation
DDB1- and CUL4-associated factor 8 plays a critical role in spermatogenesis
Xiuli Zhang, Zhizhou Xia, Xingyu Lv, Donghe Li, Mingzhu Liu, Ruihong Zhang, Tong Ji, Ping Liu, Ruibao Ren
《医学前沿(英文)》 2021年 第15卷 第2期 页码 302-312 doi: 10.1007/s11684-021-0851-8
ZnFe2O4/BiVO4 Z-scheme heterojunction for efficient visible-light photocatalytic
《化学科学与工程前沿(英文)》 2023年 第17卷 第11期 页码 1728-1740 doi: 10.1007/s11705-023-2322-z
关键词: ZnFe2O4/BiVO4 Z-scheme heterojunction photocatalytic degradation ciprofloxacin
Xiu-Liang TAO MM, Sheng-Hao TU MD, Ri-Bo XIONG MM, Yong-Hong HU BM,
《医学前沿(英文)》 2010年 第4卷 第2期 页码 220-224 doi: 10.1007/s11684-010-0025-6
关键词: arthritis rheumatoid molonuclear cells tumor necrosis factor gene polymorphisms triptolid
Li4SiO4-coated LiNi0.5Mn1.5O4 as the high performance
Shifeng YANG, Wenfeng REN, Jian CHEN
《能源前沿(英文)》 2017年 第11卷 第3期 页码 374-382 doi: 10.1007/s11708-017-0494-2
关键词: lithium-ion batteries cathode material LiNi0.5Mn1.5O4 lithium-ion conductor coating
Chaojin Jiang, Xiaoqian Jiang, Lixun Zhang, Yuntao Guan
《环境科学与工程前沿(英文)》 2020年 第14卷 第3期 doi: 10.1007/s11783-020-1224-2
关键词: 2 2′ 4 4′-tetrabromodiphenyl ether (BDE-47) Ascorbic acid Reductive debromination Zero-valent zinc
Knockdown of RFC4 inhibits the cell proliferation of nasopharyngeal carcinoma and
《医学前沿(英文)》 2023年 第17卷 第1期 页码 132-142 doi: 10.1007/s11684-022-0938-x
4D打印定律 Article
Farhang Momeni, Jun Ni
《工程(英文)》 2020年 第6卷 第9期 页码 1035-1055 doi: 10.1016/j.eng.2020.01.015
《化学科学与工程前沿(英文)》 2022年 第16卷 第7期 页码 1090-1100 doi: 10.1007/s11705-021-2091-5
关键词: synthesis pyrimidin-4-amine derivatives 1 2 4-oxadiazole insecticidal activity structure-activity relationship
Veronika BOLSHAKOVA, Annie GUERRIERO, Gilles HALIN
《工程管理前沿(英文)》 2020年 第7卷 第1期 页码 104-118 doi: 10.1007/s42524-019-0041-4
关键词: BIM 4D BIM 4D BIM use AEC project management collaboration decision making
4Pi荧光超分辨显微术综述 Review
郝翔, 李依明, 傅爽, 李旸晖, 许迎科, 匡翠方, 刘旭
《工程(英文)》 2022年 第11卷 第4期 页码 146-153 doi: 10.1016/j.eng.2020.07.028
Bin YANG MS , Xianglin YUAN , Yanmei ZOU , Qingsong XI , Guoxian LONG , Qiang FU , Guangyuan HU MM ,
《医学前沿(英文)》 2009年 第3卷 第3期 页码 303-308 doi: 10.1007/s11684-009-0060-3
对双层组件4D打印可逆性的初步研究 Article
Amelia Yilin Lee, Jia An, Chee Kai Chu, Yi Zhang
《工程(英文)》 2019年 第5卷 第6期 页码 1159-1170 doi: 10.1016/j.eng.2019.09.007
增材制造的快速发展和形状记忆聚合物材料的进步推动了四维(4D)打印技术的进展。随着设计方面的不断改进,逐渐证明有可能实现可逆4D打印或双向4D打印。这项技术将完全消除对人为干预的需要,因为编程完全由外部刺激驱动,这使得4D打印部件可以在多个周期内启动。本研究提出了一种新的可逆4D打印驱动方法,其中弹性体的溶胀和热量用于编程阶段,以及热量用于恢复阶段。本研究的主要重点在于自驱动设计这一步骤。采用这种方式理解曲率可以提供对可逆4D打印结构的高度控制。
标题 作者 时间 类型 操作
转录因子HNF1A、HNF4A和FOXA2调节肝细胞蛋白质N-糖基化
Vedrana Vičić Bočkor,Nika Foglar,Goran Josipović,Marija Klasić,Ana Vujić,Branimir Plavša,Toma Keser,Samira Smajlović,Aleksandar Vojta,Vlatka Zoldoš
期刊论文
Relationship between expression of hepatocyte grow factor and apoptosis of trophoblasts in hypertensive
OUYANG Shan, ZHANG Qinghua, QIAO Fuyuan
期刊论文
carcinoma progression by repressing translation through the interaction with eukaryotic initiation factor4A-I
期刊论文
DDB1- and CUL4-associated factor 8 plays a critical role in spermatogenesis
Xiuli Zhang, Zhizhou Xia, Xingyu Lv, Donghe Li, Mingzhu Liu, Ruihong Zhang, Tong Ji, Ping Liu, Ruibao Ren
期刊论文
Inhibition of TNF-alpha secretion from peripheral blood monocular cells by triptolid is associated withTNF-alpha-308 gene polymorphisms in rheumatoid arthritis patients
Xiu-Liang TAO MM, Sheng-Hao TU MD, Ri-Bo XIONG MM, Yong-Hong HU BM,
期刊论文
Enhanced debromination of 2,2′,4,4′-tetrabromodiphenyl ether (BDE-47) by zero-valent zinc with ascorbic
Chaojin Jiang, Xiaoqian Jiang, Lixun Zhang, Yuntao Guan
期刊论文
Synthesis, insecticidal activities and DFT study of pyrimidin-4-amine derivatives containing the 1,2,4
期刊论文
Identifying stakeholders’ roles and relevant project documents for 4D-based collaborative decision making
Veronika BOLSHAKOVA, Annie GUERRIERO, Gilles HALIN
期刊论文
Effects of hypoxia inducible factor-1alpha siRNA on the invasion of human Hela cells and expression of
Bin YANG MS , Xianglin YUAN , Yanmei ZOU , Qingsong XI , Guoxian LONG , Qiang FU , Guangyuan HU MM ,
期刊论文